Streptavidin Protein (Cy5)

Produktnummer ABIN7764574

Native Streptavidin-Protein exprimiert in Streptomyces avidinii.

Kurzübersicht für Streptavidin Protein (Cy5) (ABIN7764574)

Target: Streptavidin

Protein-Typ: Native

Spezies: Streptomyces avidinii

Quelle: Streptomyces avidinii

Applikation: Western Blotting (WB), ELISA, Fluorescence Microscopy (FM), FLISA, Flow Cytometry (FACS), Immunohistochemistry (IHC), Multiplex Assay (MA), Dot Blot (DB), Immunofluorescence (IF), Microarray (MA), Fluorescence in situ hybridization (FISH), Immunoelectron Microscopy (IEM), Functional Studies (Func)

Produktdetails

Aufreinigungstag / Konjugat: Dieses Streptavidin Protein ist gelabelt mit Cy5.

Verwendungszweck: Streptavidin Cy5 Conjugated

Aufreinigung: Cy5 conjugated Streptavidin was prepared from chromatographically purified streptavidin followed by conjugation to the dye Cy5 and extensive dialysis against the buffer stated above.   Assay by immunoelectrophoresis resulted in a single precipitin arc aga

Anwendungsinformationen

Applikationshinweise:

Flow Cytometry Dilution: 1:500 - 1:2,500

Application Note: Streptavidin Cy5 Conjugated has been tested by dot blot and western blot and is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, in

FLISA Dilution: 1:10,000 - 1:50,000

IF Microscopy Dilution: 1:1,000 - 1:5,000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Restore with deionized water (or equivalent) (in 1.0 mL)

Konzentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable Expiration date is one (1) year from date of receipt.

Haltbarkeit: 12 months

Antigendetails

Target: Streptavidin

Hintergrund:

Synonyms: SA, S avidin, streptococcus avidin, Streptavidin Cy5 Conjugated, Cy5 conjugated Streptavidin

Background: Streptavidin is isolated from bacteria, Streptomyces avidinii, and has an exceptionally high binding affinity for B7 (biotin). Rockland offers streptavidin in unconjugated and conjugated forms for common immunoassays including ELISA, western blotting, im